اثرات تغذیه‌ای دانه‌‌های روغنی کلزا و سویا آسیاب شده به عنوان مکمل چربی بر عملکرد میش‌های قزل در دوره‌ی انتقال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد تغذیه دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه، ایران

2 استادیار ، گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه، ایران

3 دانشجوی دکتری گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه، ایران

چکیده

سابقه و هدف: ماه آخر آبستنی، جزء مراحل حساس برای دام‌ها محسوب می‌شود. تامین مواد مغذی و انرژی مورد نیاز در این زمان امری حیاتی به شمار می‌آید، به‌طوری‌که عدم تامین انرژی کافی در این زمان، احتمال ابتلاء دام به بیماری‌های متابولیکی را افزایش می-دهد که می‌تواند به میزان زیادی با افزایش تراکم انرژی جیره با استفاده از دانه‌های روغنی از بروز آن پیشگیری کرد. بنابراین این آزمایش به منظور بررسی اثرات دانه‌ی کلزا و سویا بر عملکرد، تولید و ترکیبات شیر، فرآیندهای تخمیر شکمبه‌ای و متابولیت‌های خونی میش‌های قزل در دوره‌ی انتقال انجام گرفت.
مواد و روش‌ها: این آزمایش در قالب یک طرح کاملا تصادفی با استفاده از 16 راس میش قزل با میانگین سنی سه سال و میانگین وزن ‌(kg 8/3±59) و در یک دوره‌ی 74 روزه (14روز عادت‌دهی و 60 روزه ثبت صفات) انجام شد. تیمارهای آزمایشی شامل: 1- شاهد (جیره‌ی بدون مکمل چربی) 2- جیره‌ی پایه‌ی حاوی 5 درصد دانه‌ی کلزای آسیاب شده به عنوان مکمل چربی، 3- جیره‌ی پایه‌ی حاوی 5 درصد دانه‌ی سویای آسیاب شده به عنوان مکمل چربی و 4- جیره‌ی پایه‌ی حاوی مخلوط 5 درصد دانه‌ی کلزا و سویای آسیاب شده به نسبت 50:50 بود. جیره‌ها به صورت کاملا مخلوط در دو نوبت 6:00 و 18:00 در اختیار دام‌ها قرار داده می-شد. میزان افزایش وزن بره‌ها در دوره‌ی بعد از زایش، همه روزه با استفاده از باسکول دیجیتالی اندازه‌گیری شد. به منظور تعیین شیر تولیدی و ترکیب شیر میش‌ها، نمونه‌ی شیر در دو روز متوالی جمع‌آوری و با استفاده از دستگاه میلکواسکن آنالیز شد. نمونه‌ی مایع شکمبه به منظور بررسی پارامترهای تخمیر شکمبه‌ای، در روز بیستم بعد از زایش با استفاده از روش سوند مری گرفته شد. نمونه‌ی خون وریدی نیز از طریق ورید وداج در روز بیستم بعد زایش، برای اندازه‌گیری فرآسنجه‌های خونی گرفته شد.
نتایج: نتایج نشان داد که وزن تولد بره‌ها، میزان خوراک مصرفی و تغییرات روزانه‌ی وزن میش‌ها در دوره‌ی قبل و بعد از زایش تحت تاثیر تیمارهای آزمایشی قرار نگرفت(05/0>P). افزایش وزن بره‌ها تفاوت معنی‌داری بین تیمارها نشان نداد. میزان تولید شیر در تیمار مصرف کننده‌ی جیره‌ی حاوی دانه‌ی‌ کلزا به‌طور معنی‌داری بیشتر بود(05/0>P). تفاوت معنی‌داری در بررسی میزان ترکیبات شیر وجود نداشت (05/0>P). میزان تری‌گلیسرید، کلسترول و بتاهیدروکسی‌بوتیرات خون در بین تیمارهای مختلف آزمایشی تفاوت معنی-داری نداشت. مقدار گلوکز و پروتئین تام خون در دوره‌ی بعد از زایش تفاوت معنی‌داری در بین تیمارها نشان داد(05/0>P). اما میزان نیتروژن اوره‌ای خون در دوره‌ی بعد از زایش در تیمار سویا بیشتر از سایر تیمارها بود(05/0>P). تفاوت معنی‌داری در pH و میزان اسیدهای چرب فرار عمده‌ی شکمبه شامل اسید استیک، اسید پروپیونیک، اسید بوتیریک و اسید ایزووالریک اسید وجود نداشت (05/0>P). تیمار شاهد کم‌ترین میزان گوارش‌پذیری پروتئین و بیشترین میزان گوارش پذیری الیاف نامحلول در شوینده‌ی خنثی را در بین تیمارها داشت(05/0>P).
نتیجه‌گیری: با توجه به نتایج حاصل از این پژوهش، افزودن دانه‌های کلزا به عنوان مکمل چربی در جیره‌ی میش‌های قزل در دوره‌ی انتقال با کاهش تعادل منفی انرژی سبب افزایش تولید شیر بدون تاثیر منفی بر ترکیبات شیر و مصرف خوراک در سطح 5 درصد شود. هرچند نیازمند مطالعات بیشتر در این زمینه می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Nutritional effects of ground cannula and soybean as a fat supplement on performance of Ghezel ewes during the transition period

نویسندگان [English]

  • Mohammad Nazary 1
  • Younes Ali Alijoo 2
  • Hamed Khalilvandi behroozyar 2
  • Behzad Asadnejad 3
1 Urmia University
2 Urmia University
3 Urmia University
چکیده [English]

Background and objectives: The last month of pregnancy is one of the critical stages for cattle. Providing the nutrients and energy needed at this time is vital, as In sufficient energy supply at this time increases the risk of metabolic diseases in livestock, which can be greatly prevented by increasing the energy density of the diet using oilseeds. Therefore, this experiment was performed to investigate Nutritional effects of cannula and soybean as a fat supplement on performance of Ghezel ewes during the transition period
Materials and Methods: This experiment was carried in a completely randomized design using 16 ghezel ewes with an average age of three years and an average weight of ±59.8 kg in a 74-day period (14 days of adapting and 60 days of experiment). Treatments included: 1. Control (diet without fat supplementation) 2. Base diet containing 5% milled cannula as a source fat supplementation, 3. Base diet it contained 5% of milled soybean source fat supplementation and 4. Base diet containing 5% mixture of cannula and soybean at 50:50. The rations were given to the cattle in a completely mixed manner at 6:00 and 18:00. The weight gain of lambs in the postpartum period was measured daily using a digital scale. In order to determine the milk produced and the composition of ewes' milk, milk samples were collected on two consecutive days and analyzed using a Milco-scanner. Rumen fluid sample was taken to evaluate the parameters of ruminal fermentation on the twentieth day after delivery using esophageal catheter. Intravenous blood samples were taken from the venous vein on the twentieth day after delivery to measure blood counts.
Results: The results showed that the birth weight of lambs, the amount of feed intake and daily changes in the weight of ewes before and after calving were not affected by experimental treatments (p < 0.05). Weight gain of lambs did not show a significant difference between treatments. Milk production was significantly higher in the diet of cannula (p < 0.05). There was no significant difference in the amount of milk compounds (P <0.05). There was no significant difference in blood triglyceride, cholesterol and beta-hydroxybutyrate levels between different experimental treatments. The amount of glucose and total blood protein in the postpartum period showed a significant difference between treatments (p < 0.05). However, the amount of blood urea nitrogen in the postpartum period was higher in soybean treatment than other treatments (P <0.05). There was no significant difference in pH and the amount of major volatile fatty acids in the rumen including acetic acid, propionic acid, butyric acid and iso valeric acid (P <0.05). The control treatment had the lowest digestibility of protein and the highest digestibility of insoluble fibers in neutral detergent among the treatments (p < 0.05).
Conclusion: According to the results of this study, the use of oilseed cannula as a fat source at the level of 5% can improve milk production without changing the composition of milk and feed intake. However, more studies are needed in this area.

کلیدواژه‌ها [English]

  • Oilseeds
  • the transition period
  • Blood metabolites
  • Rumen fermentation
  1. Alman, P. 2014. A Meta- analysis of the effect of dietary on enteric methane production, digestibility and rumen fermentation in sheep and a comparison of these responses between cattle and sheep. journal Livestock Science.162: 97-103.
  2. Andersen, J.B., Ridder, C. and Larsen, T. 2008. Priming the cow for mobilization in the periparturient period: Effects of supplementing the dry cow with saturated fat or linseed. Journal of Dairy Science. 91: 1029–1043..
  3. Aldrich, C.G., Merchen, N.R., Drackley, J.K., Fahey, G.C. and Berger, L.L. 1997. The effects of chemical treatment ofwhole canola seed on intake, nutrient digestibilities, milk production, and milk fatty acids of Holstein cows. Journal of Animal Science. 75: 512-521.
  4. Allen, M.S., Bradford, B.J. and Harvatine, K.J. 2005. The cow as a model to study food intakeregulation. Annual Review in Nutrition. 25: 523.47.
  5. 1990. Official Methods of Analysis. 15th ed. Assoc. Office. Anal.Chem. Washington. DC.
  6. Bobe, G., Lindberg, G. L., Reutzel, L. F. and Hanigan, M. D. 2009. Effect of lipid supplementation on the yield and composition of milk from cows with different b-lactoglobulin phenotypes. Journal of Dairy Science. 92:197-203.
  7. Block, E. and Sanchez, W.K. 2000. Special nutritional needs of the transition cow. In: Porceedings of Mid-South ruminant nutrition conference. Texas Animal Nutrition Council. Dallas TX. Pp: 1-16.
  8. Brumby, P.E., Anderson, M., Tuckley, B., Storry, J.E. and Hibbit, K.G. 2000. Lipid Metabolism in the Cow during Starvation Induced Ketosis. Journal Biochemistry. 146: 609-615.
  9. Baumgard, L. H., Matitashvili, E., Corl, B.A., Dwyer, D.A. and Bauman, D. E. 2002. Trans-10, cis-12 Conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. Journal of Dairy Science. 85: 2155-2163.
  10. Chaturvedi, O.H., Bhatta, R., Verma, D.L. and Singh, N.P. 2006. Effect of flushing on nutrient utilization and reproductive performance of ewes grazing on community rangeland. Asian-Australian Jornal of Animal Science. 19: 521-525.
  11. Dehority, B.A. 2005. Effect of pH on viability of Entodinium caudatum, Entodinium exiguum, Epidinium caudatum, and Ophryoscolex purkynjei in vitro. Journal of Eukaryotic Microbiology. 52: 339-342.
  12. De Peters, E., German, J., Taylor, S., Essex, S. and Perez-Monti, H. 2001. Fatty acid and triglyceride composition of milk fat from lactating Holstein cows in response to supplemental canola Jornal of Dairy Science. 84: 929-936.
  13. Dirandeh, E., Towhidi, A., Zeinoaldini, S., Ganjkhanlou, M., Ansari pirsaraei, z. and Fouladi- Nashta, A. 2013. Effects of different polyunsaturated fatty acid supplementation during the postpartum periods of early lactating dairy cows on milk yield, metabolic responses, and reproductive performances. Journal of Animal science. 91: 713-721.
  14. Domingues, J. L., Nunaz, A. J., Gomes, R. C., Valintoe, A. C., Silva, S. L., Pereira, A.S. and Nogueira filho, J.C. 2015. Effect of high oil corn in the diets of Nellore steers on growth performance, carcass characteristics , meat quality, and longissimus muscle fatty acid profile. Livestock science. 174: 31-38..
  15. Ghoorchi, T., Gharabash, A.M. and Torbatinejad, N.M., 2006. Effect of calcium salts of long chain fatty acid on performance and blood metabolites of atabay lambs. Asian Journal of Animal and Veterinary Advances. 1: 70–75.
  16. Grummer, R.R., Mashek, G.D. and Hayirili, A. 2004. Dry matter intake and energy balance in thetransition period. Veterinary Clinics of North America, Food Animal practice. 20: 447-470.
  17. Gómez-Cortés, P., Bach, A., Luna, P., Juárez, M.and Fuente, M.A. 2009. Effects of extruded linseed supplementation on n-3 fatty acids and conjugated linoleic acid in milk and cheese from ewes. Jornal of Dairy Science.  92: 4122–4134.
  18. Hess, B.W., Moss, G.E. and Rule, D.C. 2008. A Desade of developments in the area of fat supplementation research with beef cattle and sheep. Journal of Animal Science. 86: 188-204.
  19. DaghighKia, H., Aslani Kordkandi, Gh., Moghaddam, Gh., Alijani, S. and Hosseinkhani, A. 2012. The effect of flaxseed and soybean on the diet of flushing of reproductive performance of Moghani sheep out of the breeding season. Jornal of Animal Science Resercher. 22: 173-184 (in Persian).
  20. Fukushima, D., 1991. Structures of plant storage protein and their functions. Food Reviews International. 7: 353-381.
  21. Hristov, A., Domitrovich, C., Wachter, A., Cassidy, T., Lee, C., Shingfield, K., Kairenius, P., Davis, J. and Brown, J. 2011. Effect of replacing solvent-extracted canola meal with high-oil traditional canola, high-oleic acid canola, or high-erucic acid rapeseed meals on rumen fermentation, digestibility, milk production, and milk fatty acid composition in lactating dairy cows. Jornal of Dairy Science. 94: 4057-4074.
  22. Froetschel, M.A. 2011. Nutritional intervention to improve the calcium and energetic status of highproducing transition dairy cattle. In: 25th Annual Meeting Proceedings. 2 Nov., Georgia Farm Bureau Building Macon, Southeast Dairy HerdManagement Conference, GA. Pp: 19-27.
  23. Kucuk, O., Hess, B.W. and Rule, D.C. 2004. Soybean oil supplementation of a high-concentrate dietdoes not affect site and extent of organic matter, starch, neutral detergent fiber, or nitrogen digestion, butinfluences both ruminal metabolism and intestinal flow of fatty acids in limit-fed lambs flow of fattyacids in ewes. Journal of Animal Science. 82: 2985-2994.
  24. Khorasani, G.R. and Kennelly, J.J. 1998. Effect of Added Dietary Fat on Performance, Rumen Characteristics andPlasma Metabolites of Midlactation Dairy Cows. Journal of Dairy Science. 81: 2459-2468.
  25. Kim, S.C., Adesogan, A.T., Badinga, L. and Staples, C.R. 2007. Effects of dietary n-6: n-3 fatty acid ratio on feed intake, digestibility, and fatty acid profiles of the ruminal contents, liver, and muscle of growing lambs. Jornal of Animal Science. 85: 706-716.
  26. Lough, D. S., Solomon, M. B., Rumsey, T.S., Elsasser, T. H., Slyter, L. L., Kah1, S. and Lynch, G. P. 1991. Effects of dietary canola seed and soy lecithin in high-forage diets on performance, serum lipids, and carcass. Jornal of Animal Science. 69: 3292-3298.
  27. Leupp, J.L., Lardy, G.P., Soto-Navarro, S. A., Bauer, M.L. and Caton, J.S. 2006. Effects of canola seedsupplementation on intake, digestion, duodenal protein supply, and microbial efficiency in steers fed forage-baseddiets. Journal of Animal Science. 84:499-507.
  28. 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Natl .Acad. Press, Washington, DC.
  29. Ottenstein, D.M and Batler, D.A. 1971 Improved gas chromatography separation of free. Acids C–C in dilute solution. Analytical Chemistry. 43: 952-955.
  30. Overton, T.R. and Waldron, M.R. 2004. Nutritional management of transition dairy cows; Strategies tooptimize metabolic health. Journal of Dairy Science. 87: 105-119.
  31. Onetti, S.G., Shaver, R.D., McGuire, M. A. and Grummer, R.R. 2001. Effect of type and level of dietary fat of rumen fermentation and performance of dairy cows fed corn silage-based diets. Journal of Dairy Science. 84: 2751-2759.
  32. Pires, J.A., Souza, A.H. and Grummer, R. 2007. Induction of hyper lipid emiaby intravenous infusion of tallow emulsion causes insulin resistance in Holstein Cows. Journal of Dairy Science. 90: 2735-2744.
  33. Reynolds, C.K., Aikman, P.C., Lupoli, B., Humphries, D.J. and Beever, D.E. 2003. Splanchnicmetabolism of dairy cows during the transition from late gestation through early lactation. Journal of Dairy Science. 86: 1201-1217.
  34. Russel, A.J., Doney, F.J. and Gunn, R.G. 1969. Subjective assessment of fat in live sheep. Journal of Agricultural Science, Cambridge. 72: 451–454.
  35. Reynal, S.M., Ipharraguerre, I.R., Liñ eiro, M., Brito, A.F., Broderick, G.A. and Clark, J.H. Omasal flow of soluble proteins, peptides, and free aino acids in dairy cows fed diets supplemented with proteins of varying ruminal degaradbilities. Journal of Dairy Science. 90: 1887-1903.
  36. Smith, B.P. 1996. Large animal internal medicine (2nd). Mosby press.
  37. Santos, J.E.P.,Bilby, T.R., Thatcher, W.W., Staples, C.R. and Silvestre. F.T. 2008. Long chain fatty acid od diet as factors influencing reproduction in cattle. Reproduction in Domestic Animals. 43: 23-30.
  38. SAS Institute. 2003. STAT user's guide: Statistics. Version 9.1. Cary, NC: Statistical Analysis System Institute.
  39. Solomon, M. B., Lynch, G. P., Paroczay, E. and Norton, S. 1991. Influence of rapeseed meal, whole rapeseed, and soybean meal on fatty acid composition and cholesterol content of muscle and adipose tissue from ram lambs. Journal of Animal Science. 69: 4055-4061.
  40. Titi, H.H. and Kridli, R.T. 2008. Reproductive performance of seasonal ewes fed dry fat source during their breeding season. New Zealand Journal of Agricultural Research. 51: 25-32.
  41. Van Keulen, J. and Young, B. A. 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science. 44: 282–287.
  42. Yang, W.Z., Benchaar, C., Ametaj, B. N., Chaves, A.V., He, M. L., and McAllister, T.A. 2007. Effects of garlic and juniper berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows. Journal of Dairy Science. 90: 5671–5681.
  43. Zened, A., Enjalbert, F., Nicot, M. C. and Troegeler-Meynadier, A. 2013. Starch plus sunflower oil addition to the diet of dry dairy cows results in a trans-11 to trans-10 shift of biohydrogenation. Journal of Dairy Science. 96: 451-459.