فراتحلیل ارتباط چندشکلی ژن‌های DGAT1 و لپتین با برخی صفات کمی و کیفی تولید شیر در گاوهای شیری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد ، گروه علوم دامی، دانشکده کشاورزی و محیط زیست، دانشگاه اراک، اراک، ایران

2 استادیار، گروه علوم دامی، دانشکده کشاورزی و محیط زیست، دانشگاه اراک، اراک، ایران

3 استادیار ، گروه علوم دامی، دانشکده کشاورزی و محیط زیست، دانشگاه اراک، اراک، ایران

چکیده

چکیده
سابقه و هدف: فراتحلیل (متاآنالیز) روشی آماری برای یکی کردن نتایج حاصل از تحقیقات مستقل با فرضیه های مشابه است که در تحقیقات دامی می تواند منجر به افزایش توان تجزیه آماری و نیز بالا بردن تکرارپذیری نتایج، نسبت به تحقیقات انفرادی شود. چندشکلی ژن های بزرگ اثر دی آسیل گلیسرول آسیل ترانسفراز 1 (DGAT1) و لپتین در طی سال های اخیر به خاطر اثرات احتمالی آن ها روی صفات مهم اقتصادی در تحقیقات مختلف، توسط محققین علوم دامی مورد توجه قرار گرفته است. با این وجود، ارتباط بین این ژن ها و صفات مرتبط با تولید شیر در نژادهای مختلف گاو شیری متناقض، یعنی گاهی معنی دار و گاهی نیز بدون ارتباط معنی دار گزارش شده است. هدف از پژوهش حاضر، بررسی اثرات کلی ژن های DGAT1 و لپتین روی برخی از صفات مهم اقتصادی شامل میزان تولید شیر، مقدار و درصد چربی و پروتئین شیر در گاوهای شیری به روش فراتحلیل بود.
مواد و روش ها: در این پژوهش، از مطالعات منتشر شده در مجلات و پایان نامه های معتبر تا سال 2018 استفاده شد. پس از انجام مراحل مختلف کنترل کیفیت، به ترتیب از 9 و 4 تحقیق گزارش شده برای جایگاه های K232A و Sau3AI مربوط به ژن های DGAT1 و لپتین، استفاده شد. این بررسی با استفاده از نرم افزار Stata v. 15انجام شد. شاخص عدم تجانس (I2) برای همه پارامترها معنی دار بود، بنابراین از مدل اثرات تصادفی استفاده شد. داده ها برای هر متغیر با استفاده از این مدل ها، برای تخمین اندازة اثر، دامنه اطمینان 95 درصد و سطح معنی داری آماری، آنالیز شدند. تمام متغیرها در این مطالعه به صورت داده های پیوسته بودند. اندازة اثر برای داده‌های پیوسته، تفاوت میانگین استانداردشده بود که برای چندشکلی K232A ژن DGAT1 و چندشکلی Sau3AI ژن لپتین، روی مقدار و درصد چربی و پروتئین، و مقدار تولید شیر محاسبه شد.
یافته ها: نتایج این تحقیق نشان داد که چندشکلی جایگاه ژنی K232A در ژن DGAT1 اثر معنی داری روی درصد و مقدار چربی شیر داشته و می تواند نقش مهمی در افزایش مقدار آن ها ایفا کند، اما ارتباط آن با درصد و مقدار پروتئین و همچنین میزان تولید شیر معنی دار نیست. همچنین نتایج بررسی چندشکلی جایگاه ژنی Sau3AI ژن لپتین نشان داد که این جایگاه با هیچ یک از صفات مورد بررسی ارتباط معنی داری ندارد.
نتیجه گیری: در مجموع، نتایج حاصل از این تحقیق با شناسایی اثرات کلی ژنوتیپ‌های مورد مطالعه ژن های DGAT1 و لپتین روی صفات مرتبط با تولید شیر در گاوهای شیری، می تواند نقش مهمی در برنامه ریزی تحقیقات بعدی در این زمینه داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

A meta-analysis for association between DGAT1 and Leptin genes polymorphisms with some milk production traits in dairy cows

نویسندگان [English]

  • Marjan Ghorbani 1
  • Mohammad Hossein Moradi 2
  • AmirHossein Farahani 3
  • Mehdi Mirzaei 3
1 Department of Animal Science, Arak University, Arak, Iran
2 Department of Animal Science, Arak University, Arak, Iran
3 Department of Animal Science, Arak University, Arak, Iran
چکیده [English]

Abstract
Background and objectives: Meta-analysis is a statistical approach for combining the results from independent studies with the same assumptions, that is able to increase the statistical analysis power and the repeatability of the results in the livestock researches, comparing to single-study analysis. The polymorphism in the major genes of Diacylglycerol O-Acyltransferase 1 (DGAT1) and Leptin have drawn much attention from animal scientists during recent years, for their possible roles in the economically important traits. However, the relationships between these genes and milk production traits have been incompatible in dairy cows, it means sometimes significant and some other times no significant association has been reported. The purpose of this study was to investigate the overall effect of DGAT1 and Leptin genes on some economically important traits including milk yield, fat and protein content and percentages in dairy cows by using meta-analysis.
Materials and methods: in this study, all researches published in the validated journals and thesis up to 2018 were used. Following to quality control, 9 and 4 studies reported for K232A and Sau3AI polymorphisms of DGAT1 and Leptin genes were used, respectively. The analysis was carried out using the Stata v. 15. Non conformance index (I 2) was significant for all parameters, so that a random risk model was used. Data were analyzed for each variable using these models for measuring the effect size, 95% confidence interval and statistical significance level of the measurements. All variables were continuously in this study. The measurement of the effect size for continuous data was carried out using the standardized mean difference (SMD) that calculated for the polymorphisms of K232A and Sau3AI loci in DGAT1 and Leptin genes on the milk yield, fat and protein content and percentage.
Results: the results of this study showed that the polymorphism in the K223A locus of DGAT1 gene, has a significant effect on lipid content and percentage, and can play an important role in increasing of their amounts, but its relationship with milk yield and protein content and percentage is not significant. Also, the results for Sau3AI polymorphism in the Leptin revealed that this locus had no significant effect on the traits investigated in this study.
Conclusion: Overall, the results of this study with identifying the overall effects of the studied genotypes of DGAT1 and Leptin genes on milk production traits in dairy cows can play an important role for planning next researches in this field.

کلیدواژه‌ها [English]

  • Polymorphism
  • Leptin
  • Diacylglycerol O-Acyltransferase 1 (DGAT1)
  • Dairy cattle

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نشریه پژوهش‌ در نشخوار کنندگان
دوره 9، شماره 1
خرداد 1400
صفحه 17-36

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