Effect of feeding Bacillus coagulans and Bacillus subtilis probiotics on growth performance, health and blood parameters in suckling Holstein calves

Authors

Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abstract

Effect of feeding Bacillus coagulans and Bacillus subtilis probiotics on growth performance and blood parameters in suckling Holstein calves

Extended abstract

Background and purpose: Gastrointestinal tract infections and subsequent diarrhea are the main reason of mortality of newborn calves. Diarrhea is the most common disease that occurs in cattle farms and causes death and serious economic losses to the livestock industry. Due to negative effects of antibiotics, including Bacterial resistance to antibiotics, many countries in the world have banned the use of these antibiotics in the animal industry since 1996. The use of probiotics is mostly seen as an alternative to antibiotics in animals, and many scientific works show the beneficial effects of supplements with probiotic strains in the diets of different farm animals. Probiotics have been shown to have positive effects on the whole body, including weight gain and immune function, and reducing the presence of pathogens. This study aimed to evaluate the probiotics Bacillus coagulans and Bacillus subtilis and their effect on the growth performance, health, and prevalence of diarrhea in suckling Holstein calves. . . . . . .

Materials and methods: The present study was conducted in the form of a completely randomized design with 32 replications in two treatments. Calves were monitored for 28 days. Body weight was measured every week, and dry matter intake and health status were checked and measured every day. Blood samples were taken from the calves on the first, seventh, 14th, 21st, and 28th days, and the blood was kept at 4°C until reaching the laboratory, and then blood parameters were measured. fecal samples were taken for microbial culture on the 7th, 14th, and 28th days and were cultured in the laboratory.

Findings: The results showed that probiotic supplementation does not affect weight, daily weight gain, skeletal growth, Feed Efficiency, and hematological parameters. However, feed intake and the number of Lactobacillus in feces increased significantly (P < 0.05). Additionally, probiotic supplementation reduced fecal score, diarrhea duration, and body temperature significantly (P<0.05).. . .

Conclusion: Probiotic oral supplement improved the symptoms of diarrhea and the negative effects of diarrhea, and it also improved the performance of feed consumption and increased the beneficial bacteria of the gastrointestinal tract system in suckling calves. By preventing diarrhea, probiotic supplements can reduce the losses caused by calves dying and, as a result, make dairy farms more profitable.

Keywords: calf health, diarrhea, prebiotic, probiotic, suckling calf

Keywords

Main Subjects

References

Alugongo, G., Xiao, J., Chung, Y., Dong, S., Li, S., Yoon, I., Wu, Z. and Cao, Z. 2017. Effects of Saccharomyces cerevisiae fermentation products on dairy calves: Performance and health. Journal of Dairy Science, 100: 1189-1199.
Azizi Nasab, F., Bayat Kouhsar, J., Ghanbari, F., Rahchamani, R. and  Halakoo, G. 2020. Blood metabolites, Dairy calves, Performance, Probiotic, Skeletal growth indices. Journal of Ruminant Research, 8(1), 77-94.(In Persian).
Britton, R. A. and Versalovic, J. 2008. Probiotics and gastrointestinal infections. Interdisciplinary perspectives on infectious diseases, 2008: 126-129.
Dibaji, S. M., Seidavi, A., Asadpour, L. and da Silva, F. M. 2014. Effect of a synbiotic on the intestinal microflora of chickens. Journal of Applied Poultry Research, 23: 1-6.
Jasper, J. and Weary, D. 2002. Effects of ad libitum milk intake on dairy calves. Journal of Dairy Science, 85: 3054-3058.
Jenny, B., Vandijk, H. and Collins, J. 1991. Performance and fecal flora of calves fed a Bacillus subtilis concentrate. Journal of Dairy Science, 74: 1968-1973.
Karamzadeh-Dehaghani, A., Towhidi, A., Zhandi, M., Mojgani, N. and Fouladi-Nashta, A. 2021. Combined effect of probiotics and specific immunoglobulin Y directed against Escherichia coli on growth performance, diarrhea incidence, and immune system in calves. Animal, 15: 100124.
Khajeh Bami, M., Afsharmanesh, M. and Ebrahimnejad, H. 2020. Effect of dietary Bacillus coagulans and different forms of zinc on performance, intestinal microbiota, carcass and meat quality of broiler chickens. Probiotics and Antimicrobial Proteins 12: 461-472.
Liu, B., Wang, C., Huasai, S., Han, A., Zhang, J., He, L. and Aorigele, C. 2022. Compound Probiotics Improve the Diarrhea Rate and Intestinal Microbiota of Newborn Calves. Animals, 12: 322.
Lucey, P., Lean, I., Aly, S., Golder, H., Block, E., Thompson, J. and Rossow, H. 2021. Effects of mannan-oligosaccharide and Bacillus subtilis supplementation to preweaning Holstein dairy heifers on body weight gain, diarrhea, and shedding of fecal pathogens. Journal of Dairy Science 104: 4290-4302.
McGuirk, S. M. and Peek, S. F. 2014. Timely diagnosis of dairy calf respiratory disease using a standardized scoring system. Animal Health Research Reviews, 15: 145-147.
Mingmongkolchai, S. and Panbangred, W. 2018. Bacillus probiotics: an alternative to antibiotics for livestock production. Journal of Applied Microbiology, 124: 1334-1346.
Molyanova, G., Nogotkov, M. and Chigina, N. 2021. Effect of the Bacillus subtilis-based drug on the morphobiochemical and productive parameters of calves. E3S Web of Conferences, 273: 02011: EDP Sciences.
Renaud, D., Kelton, D., Weese, J., Noble, C. and Duffield, T. 2019. Evaluation of a multispecies probiotic as a supportive treatment for diarrhea in dairy calves: A randomized clinical trial. Journal of Dairy Science, 102: 4498-4505.
Riddell, J., Gallegos, A., Harmon, D. and Mcleod, K. 2010. Addition of a Bacillus based probiotic to the diet of preruminant calves: Influence on growth, health, and blood parameters1, 2, 3. The International Journal of Applied Research in Veterinary Medicine, 8: 78-85.
Signorini, M., Soto, L., Zbrun, M., Sequeira, G., Rosmini, M. and Frizzo, L. 2012. Impact of probiotic administration on the health and fecal microbiota of young calves: a meta-analysis of randomized controlled trials of lactic acid bacteria. Research in Veterinary Science, 93: 250-258.
Studds, M. J., Deikun, L. L., Sorter, D. E., Pempek, J. A. and Proudfoot, K. L. 2018. Short communication: The effect of diarrhea and navel inflammation on the lying behavior of veal calves. Journal of Dairy Science, 101: 11251-11255.
Szczerbiec, D., Piechocka, J., Głowacki, R. and Torzewska, A. 2022. Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis. In Molecules, 27: 5557.
Timmerman, H. M., Mulder, L., Everts, H., Van Espen, D., Van Der Wal, E., Klaassen, G., Rouwers, S., Hartemink, R., Rombouts, F. and Beynen, A. 2005. Health and growth of veal calves fed milk replacers with or without probiotics. Journal of Dairy Science, 88: 2154-2165.
Wang, H., Yu, Z., Gao, Z., Li, Q., Qiu, X., Wu, F., Guan, T., Cao, B. and Su, H. 2022. Effects of compound probiotics on growth performance, rumen fermentation, blood parameters, and health status of neonatal Holstein calves. Journal of Dairy Science, 105: 2190-2200.
Windschitl, P. M., Randall, K. M. and Brainard, D. J. 1991. Growth performance of Holstein dairy calves supplemented with a probiotic. School of Agriculture and Land Resources Management, Agricultural, Research Progress Report 22.
Zhang, R., Zhou, M., Tu, Y., Zhang, N., Deng, K., Ma, T. and Diao, Q. 2016. Effect of oral administration of probiotics on growth performance, apparent nutrient digestibility and stress‐related indicators in Holstein calves. Journal of Animal Physiology and Animal Nutrition, 100: 33-38.
Journal of Ruminant Research
Volume 11, Issue 3
November 2023
Pages 81-96

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