The effect of different additives (organic acid, mollasses and enzyme) on chemical composition, aerobic stability, fermentation parameters, digestibility and gas production of pea (Pisum sativum) silage

Authors

1 Master student, Department of Animal Science, Faculty of Agriculture and Natural Resources, Gonbad Kavos University,

2 Assistant Professor, Faculty of Agriculture and Natural Resources, Gonbad Kavos University,

3 Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University

Abstract

Background and Objectives: Sources of protein are the most expensive part of animal feed, and every year a large amount of these sources are imported from abroad for use in animal husbandry industry. One of the important steps in reducing the cost of feed is the optimal use of available resources and the recognition of new foods and their use in animal rations. On the other hand, every year, a huge amount of agricultural product residues are obtained, which can be used in animal nutrition. Optimum use of agricultural by-products and processing industries in feeding ruminants is important from economic and environmental aspects. Pea (Pisum sativum L.) is a cool-season crop and one of the most important legumes, in the temperate climatic regions and it has been widely consumed as a legume or vegetable throughout the world for the purpose of both human nutrition and animal nutrition. Peas are cultivated with the aim of producing green seeds, but after harvesting the pods containing seeds, its waste can be used by ruminants as high-quality bulk feed. The by-product after the harvesting of chickpeas (green chickpeas) which can be extracted and separated manually is the parts of the stem, leaves and pods that are free of green seeds. This research was conducted in order to investigate the effect using organic acid (acetic acid) and fibrinolytic enzyme and molasses on chemical composition, fermentation characteristic, gas production and digestibility parameters of pea silage.
Materials and Methods: Whole crop pea silage was harvested and chopped with a conventional forage harvester under farm condition to length of 2-3 cm. Representative of pea forage sample was packed manually, in triplicate into plastic bags. The filled silos were stored at ambient temperature and allowed to ensile for 45 days. The following treatments were applied to the forage samples: 1) pea forage without any additives (control), 2) control + fibrinolytic enzyme(2 grams per kg of dry matter), 3) control + organic acid(2%), 4) control + molasses(8%) and 5) control + (fibrinolytic enzyme+ molasses). Additives diluted in deionized water and applied with a hand held sprayer while forage samples were stirred manually. A similar quantity of deionized water was sprayed on the control forage. After designated ensiling times, silos were opened and the ensiled forage was mixed thoroughly and then were dried at a 60°C in oven for 48 h and then ground to pass through a 2 mm screen for later analysis.
Results: Results showed that there were significant differences among treatments on NDF, CP and pH (P>0.05). Enzyme treated silages had lowest NDF content (46.50%) compared with other treatments (P<0.05). The lowest pH was related to treatment containing molasses. silage treated with organic acid improved aerobic stability, significantly. There were significant differences among treatments on gas production parameters
(P<0.05) and molasses and enzyme treated pea silage had highest and lowest gas production potential than others (199.1 and 183.3 ml/g DM, respectively). Results showed that there were no significant differences among treatments on DMD and partitioning factor (P>0.05). Organic acid treated silages had lowest effect on DMD and OMD compared with other treatments (P<0.05).
Conclusion: Overall, results showed that using molasses and molasses + enzyme as additive can be improwed nutritive value of pea silage better than others. Anyway, green pea forage according to the appropriate level of crude protein similar to corn forage and its silage characteristics, has the potential of silage and can be used in livestock feeding.

Keywords

Main Subjects


Abarghoei, M., Rouzbehan, Y. and Alipour, D. 2011. Nutritive Value and Silage Characteristics of Whole and Partly Stoned Olive Cakes Treated with Molasses. Journal of Agricultural Science and Technology, 13: 709-716. (In Persian).
Aksu, T., Baytok, E., Karsli, M.A. and Muruz, H. 2006. . Effects of Formic Acid, Molasses and Inoculants Additives on Corn Silage Composition, Organic Matter Digestibility and Microbial Protein Synthesis in Sheep. Small Ruminant Reserch, 61: 29-33.
Arbabi, S., Ghorchi, T. and Naserian, A.A. 2008. The effect of dried citrus pulp, dried beet sugar pulp and wheat straw as silage additives on by-products of orange silage. Asian Journal of Animal Sciences, 2(2): 35-42.
Ashbell, G., Weinberg, Z.G., Azriel, A., Hen, Y. and Horev, B. 1991. A simple system to study the aerobic deterioration of silages. Canadian Agriculture and Engineering, 33: 391-393.
Ayubi Far, M., Gharebash, A., Bayatkouhsar, J. and Farivar, F. 2021. The effect of using different additives on chemical composition, parameters of gas production and digestibility of artichoke silage. Ruminant Research Journal, 9: 3.1-24. (In Persian).
Balabanli, C., Albayrak, S., Turk, M. and Yuksel, O. 2010. A research on determination of hay yields and silage qualities of some vetch + cereal mixtures. Turkish Journal of Field Crops, 15(2): 204-209.
Blummel M. and Becker K. 1997. The degradability characteristics of fifty-four roughages and roughage neutral-detergent fibers as described by in vitro gas production and their relationship to voluntary feed intake. British Journal of Nutrition, 77: 757-768.
Blümmel, M. and Ørskov, E.R. 1993 Composition of In vitro gas production and nylon bag degradability of roughages in predicting food intake in cattle. Animal Feed Science and Technology, 40: 109-119.
Bouriako, I.A., Shihab, H., Kuri, V. and Margerison, J.K. 2001. Influence of wilting time on silage compositional quality and microbiology of grass clover mixtures. Proceedings of the British Society of Animal Science, 88: 102-108.
Broderik, G.A. and Kang, J.H. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 63: 64-75
Buxton, D.R., Muck, R.E. and Harrison, J.H. 2003. Silage Science and Technology. American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc. Publications, Madison, WI.
Dean, D.B., Adesogan,A.T., Krueger, N. and Littell, C. 2005a. Effect of fibrolytic enzymes on the fermentation characteristics, aerobic stability, and digestibility of bermudagrass silage. Journal of Dairy Science, 88: 994-1003.
Denek, N. and Can, A. 2006. Feeding value of wet tomato pomace ensiled with wheat straw and wheat grain for Awassi sheep. Small Ruminant Research, 65(3): 260-265.
Ely, L. 1978. In fermentation of silage–a Review (M E. Mc Cullough, ed). Journal of National Feed Ingredients Association, 235-280.
Elzebroek, T. and Wind, K. 2008. Guide to cultivated plants. CAB International, Oxfordshire, UK.
Fallah, S., Susan, B. and Ali, A.S. 2014. Evaluation of competitive and economic indices in canola and pea intercropping at different rates of nitrogen fertilizer. Journal of Agroecology, 6(3): 571-581. (In Persian).
FAO. FAOSTAT statistical database. Rome. 2015. Available from: URL, faostat.fao.org.
Getachew, G., Blummel, M., Makkar, H.P.S. and Becker, K. 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds. a review. Animal Feed Science and Technology, 72: 261-281.
Getachew, G., Depiters, E.J. and Robinson, P.H. 2002. In vitro gas production provides effective method for assessing ruminant feeds. California Agri, 58: 54-58.
Hedayati poor, A., Khorvash, M., Ghorbani, Gh.R. and Al-Modarres, A. 2012. Comparison of chemical characteristics and degradability of kinds of forage and silage sorghum by corn silage in In vitro and nylon bag. Iran Journal Animal Science Research, 4: 224-232. (In Persian).
Higginbotham, G.E., Mueelier, S.C., Collar, C.A., Schultz, T.A., Brazle, F.K. and  Bush, L.J. 2004. Effects of an enzyme addition on corn silage fermentation. The Professional Animal Scientist, 10 (4): 163-168.
Kavian, A., Abbasi, A., Gharehbash, A., Pasandi, M. and Kamali, R. 2005. Pea production waste and its feeding value (chemical composition and In vivo digestibility). 2nd national simposium on losser of agricultural product. 662-674.
Kennedy, S.J. 1990. Comprison of the fermentation quality and nutritive value of sulphuric and formic acid-treated silages feed to beef cattle. Grass and forage Science, 45: 17-28.
Kung, L. and Ranjit, N.K. 2001. The effect of Lactobacillus buchneri and other additives on fermentation and aerobic stability of barley silage. Journal of Dairy Science, 84: 1149-1155.
Kung, L., Stokes, M.R. and Lin, C.J. 2003. Silages additives. Page 305 in D. R. Buxton, R. R. Muck and J. R. Harrison, eds. Silage science and technology. Agronomy monograph, No. 42, Madison, WI.
McDonald P., Henderson A.R., Heron S.J.E. 1991. The Biochemistry of Silage. Chalcombe Publications, 13 Highwoods drive, Marlow Bottom, Marlow, Bucks, and UK.
Menke, K.H. and Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Journal Animal Research Development, 28: 7-55.
Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science, 93: 217-222.
Muck, R. E., Moser, L.E. and Pitt, R.E. 2003. Post harvest factors affecting ensiling. Pages 251-304 in Silage Science and Technology. D. R. Buxton, R. E. Muck. and J. H. Harrison, ed. American Society of Agronomy, Inc., Crop Science Society of America, Inc., Soil Science Society of America, Inc. Publications, Madison, WI.
Muck, R.E. and Bolsen, K.K. 1991. Silage preservation and silage additive products. Page 105 in Field Guide for Hay and Silage Management in North America. K. K. Bolsen, J. E. Baylor, and M. E. McCullough, eds. Natl. Feed Ingred. Assoc., West Des Moines, IA.
Muck, R.E. and Pitt, R.E. 1993.Proceedings of National Silage Production Conference, Syracuse, New York, p. 55-56.
National Research Council (NRC). 2001. Nutrient requirements of dairy cattle. 7th ed. National Academy Press, Washington, DC, USA.
Nie, H., Wang, Z., You, J., Zhu, G., Wang, H. and Wang, F. 2020. Comparison of in vitro digestibility and chemical composition among four crop straws treated by Pleurotus ostreatus. Asian-Australian Journal of Animal Sciences, 33: 24-34.
Olivera, R.M. 1998. Use of in vitro gas production technique to assess the contribution of both soluble and insoluble fractions on the nutritive value of forages. A thesis submitted to the University of Aberdeen, Scotland, in partial fulfilment of the degree of Master of Science in Animal Nutrition.
Ozkul, H., Kilic, A. and Polat, M. 2011. Evaluation of mixtures of certain market wastes as silage. Asian-Australasian Journal of Animal Sciences, 24(9): 1243-1248.
Pahlow, G., Muck, R.E., Driehuis, F., Elfrink, S.J. and Spolestra, S. 2003. Microbiology of Ensiling. In: Buxton. Eds. D. R., Muck, R, E and Harrison, J. H., Silage Science and Technology. ASA.
Paya, H., Taghizadeh. A., Janmohammadi, H. and Moghadam, G.A. 2007. Nutrient Digestibility and Gas production of some Tropical Feeds Used in Ruminant Diets Estimated by the in vivo and in vitro Gas production Techniques. American Journal of Animal and Veterinary Sciences, 2(4): 108-113.
Pettersson, K. and Lindgren, S. 1989. The influence of the carbohydrate fraction and additives on silage quality. Grass and Forage Science, 45: 223.
Phllip, L.E., Underhill, L. and Garino, H. 1990. Effects of treating Lucerne with an inoculums of lactic acid bacteria or formic acid upon chemical changes during fermentation, and upon the nutritive value of the silage for lambs. Grass and Forage Science, 45: 337-348.
Pitt, R.E., Liu, Y. and Muck R.E. 1991. Stimulation of the effect of additives on aerobic stability of alfalfa and corn silages. American Society of Agricultural and Biological Engineers, 34:1633-1641.
Sandoval Castro, C.A., MagaSevilla, H., Capetillo Leal, C. and DeBHovell, F.D. 2000. Comparison of charcoal and polyethylene glycol (PEG) for neutralizing tannin activity with an in vitro gas production technique. An EAAP Satellite symposium. Wageningen International Conference Centre.The Netherlands, 18-19August: 109-110.
Saravani, M., Jalilund, Gh., Shojaian, K., Youssef Elhahi, M. and Hormazipur, H. 2010. Determining the nutritional value of Oyarsalam fodder ensiled with urea and molasses using the gas production test. The 4th Congress of Animal Sciences of Iran. University of Tehran, 1389. (In Persian).
Sheperd, A.C. and Kung Jr, L. 1996. An enzyme additive for corn silage: effects on silage composition and animal performance. Journal of Dairy Science, 79: 1760-1766.
 Sommart, K., Parker, D.S., Rowlinson, P. and Wanapat, M. 2000. Fermentation characteristics and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asian-Australian Journal Animal Science, 13: 1084-1093.
 Stokes, M.R. and Chen, J. 1994. Effects of enzyme inoculant mixture on the course of fermentation of corn silage. Journal of Dairy Science, 77:3401.
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48: 185-197.
Thomas, P., Chamberlain, D., Kelly, N. and Wait, M. 1980. The nutritive value of silages digestion of nitrogenous constituents in sheep receiving diets of grass silage and grass silage and barley. British Journal of Nutrition, 43(3):469-479.
Valizadeh, R., Abbas Ali, N. and Abtain, A.F. 2003. Biochemistry of silage. Publications of Ferdowsi University of Mashhad. Page 413.
Van Soest, P.J. 1994. Nutritional Ecology of the Ruminants, 2th Edition, Comstock Cornell University Press, USA.
Weinberg, Z.G., Ashbell, G., Hen, Y., Azrieli, A., Szakkacs, G. and Filya, I. 2002. Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri. Journal of Industrial Microbiology and Biotechnology, 28: 7-11.
Wolin, M. J. 1988. A theoretical rumen fermentation balance. Journal of Dairy Science, 43:1452-1459.
Yokota, H., Kim, J.H., Okajima, T. and Ohshima, M. 1992. Nutritional quality of Wilted napier grass ensiled with or without molasses. Asian-Australian Journal Animal Science, 5: 673.