Effect of injection of vitamin C and copper on milk production and composition, and body condition score of transition dairy cows under heat stress

Authors

1 Department of Animal Science, Ilam University, Ilam, Iran.

2 Faculty member, Ilam University

3 Assistant Prof. Animal Science Research Department, Ilam Agricultural and Natural Resources Research and Education Center, AREEO, Ilam, Iran

4 Department of Animal Science, Ilam University, Ilam, Iran

Abstract

Background and objectives: Transition period is the period between 3 weeks before and 3 weeks after parturition which is one of the most critical physiological stage in dairy cows. Decreasing dry matter intake (DMI) and negative energy balance (NEB) potentially weakens immunity system and increases higher incidence of metabolic and infectious diseases such mastitis. High temperature in summer results in heat stress which subsequently reduce DMI and changes metabolic reaction in lactating dairy cows. Consumption of some vitamins and minerals could reduce negative effects of transition period and heat stress such as vitamin C and copper. The aim of this experiment was to study the effect of injection of vitamin C and copper to transition dairy cows on milk production and composition and body condition score (BCS), DMI in calving day and blood cells in summer season.
Materials and methods: In this experiment twenty multiparous (second parity, with 603.18 ± 51 kg average body weight) and twenty multiparous (third and fourth parity, 669.05 ± 53 kg average body weight) Holstein cows were used in a completely randomized block design with 2*2 factorial arrangement. Experiment extended from 40 d before expected calving till 90 d after calving. Experimental treatments consisted of control (injection of 7 ml of NaCl % 0.9), Vitamin C (injection of 25 mg vitamin C solution/kg body weight), Copper (injection of 75 mg copper solution/day) and Vitamin C-Copper (simultaneous injection of 25 mg vitamin C solution/ kg body weight and 75 mg copper solution/day). Solutions were injected on 20 and 40 d before expected calving, calving day and 20 d after calving. Maximum temperature-humidity index during the experiment was between 68.27 to 78.20. Body condition score (BCS) changes, DMI in calving day, milk production and composition and blood cells were evaluated.
Results: Results showed that BCS, DMI in calving day, milk production and composition on d 7, 30, 60 and 90 after calving and cell blood count except of neutrophil count were not influenced by simultaneous injection of vitamin C and copper solutions. Blood neutrophil count on d 20 before expected calving did not affected by injection of vitamin C while as vitamin C injection enhanced blood neutrophil count on calving day, 10, 20 and 30 d after calving (p < 0.05). Copper solution injection in compare to lack of injection tended to less changes on BCS form calving day till 30 d after calving (P=0.06). Red blood cell counts on calving day in cows receiving copper solution injection were greater in compare to lack of injection (p < 0.05).
Conclusion: Although, individual injection of vitamin C and copper to transition dairy cows resulted in some changes in blood cell counts but simultaneous injection of vitamin C and copper to heat loaded transition dairy cows had no considerable effect on milk composition and production of the first 90 d calving.

Keywords


  1. Adin, G., Gelman, A., Solomon, R., Flamenbaum, I., Nikbachat, M., Yosef, E., Zenou, A., Shamay, A., Feuermann, Y., Mabjeesh, S.J. and Miron, J. 2009. Effects of cooling dry cows under heat load conditions on mammary gland enzymatic activity, intake of food water, and performance during the dry period and after parturition. Livest. Sci. 124: 189–195.
  2. Aggarwal, A. and Upadhyay, R. 2013.Heat Stress and Immune Function. In: Heat Stress and Animal Productivity. 1st Edition. Springer Publication, London, UK. Pp: 113-136.
  3. Allen, M.S. and Bradford, B.J. 2009. Control of eating by hepatic oxidation of fatty acids. A note of caution. J. Appetite. 53: 272-273.
  4. Association of Official Analytical Chemists. 2007. Official methods of analysis. 18th Edition. AOAC, Gaithersburg, MD, USA.
  5. Bernabucci, U., Basirico, L., Morera, P., Dipasquale, D., Vitali, A., Piccioli Cappelli, F. and Calamari, L. 2015. Effect of summer season on milk protein fractions in Holstein cows. J. Dairy. Sci. 98: 1815–1827.
  6. Campbell, M.H., Miller, J.K. and Schrick, F.N. 1999. Effect of additional cobalt, copper, manganese, and zinc on reproduction and milk yield of lactating dairy cows receiving bovine somatotropin. J. Dairy. Sci. 82: 1019–1025.
  7. Capuco, A.V., Akers, R.M. and Smith, J.J. 1997. Mammary growth in Holstein cows during the dry period: quantification of nucleic acids and histology. J. Dairy. Sci. 80: 477–487.
  8. Cerone, S.I., Sansinanea, A.S., Streitenberger, S.A., Garcia M.C. and Auza. N.J. 1998. The effect of copper deficiency on the peripheral blood cells of cattle. Vet. Res. Commun. 22: 47-57.
  9. Chase, C.R., Beede, D.K., Van Horn, H.H., Shearer, J.K., Wilcox, C.J. and Donovan,G.A. 2000. Responses of lactating dairy cows to copper source, supplementation rate, and dietary antagonist (Iron). J. Dairy. Sci. 83: 1845–1852.
  10. Collier, R.J., Doelger, S.G., Head, H.H., Thatcher, W.W. and Wilcox, C.J. 1982. Effects of heat stress during pregnancy on maternalhormone concentrations, calf birth weight and postpartum milk yield of Holstein cows. J. Anim. Sci. 54: 309–319.
  11. Cusack, P.M.V., McMeniman, N.P. and Lean, I.J. 2005. The physiological and production effects of increased dietary intake of vitamins E and C in feedlot cattle challenged with bovine herpesvirus. J. Anim. Sci. 83: 2423-2433.
  12. Du Preez, J.H., Giesecke, W.H. and Hattingh, P.J. 1990. Heat stress in dairy cattle and other livestock under Southern African conditions. Temperature-humidity index mean values during the four main seasons. J. Vet. Res. 57: 77-86.
  13. Engle, T.E., Spears, J.W., Xi, L. and Edents, F.W. 2000. Dietary copper effects on lipid metabolism and circulating catecholamine concenteration in finishing steers. J. Anim. Sci. 78: 2737- 2744.
  14. Engle, T.E., Fellner, V. and Spears, J.W. 2001. Copper status, serum cholesterol and milk fatty acide profile in Holestein cows fed varying concenteration of copper. J. Dairy. Sci. 84: 2308- 2313.
  15. Erb, C., Staudt, N., Flammer, J. and Nau, W. 2004. Ascorbic acid as a free radical scavenger in porcine and bovine aqueous humour. Ophtalmic. Res. 36: 38-42.
  16. Gao, S.T., Guo, J., Quan, S.Y., Nan, X.M., Fernandez, M.V.S., Baumgard, L.H. and Bu, D.P. 2017. The effects of heat stress on protein metabolism in lactating Holstein cows. J. Dairy. Sci. 100: 5040–5049.
  17. Garcı´a-Ispierto, I., Lo´pez-Gatius, F., Bech-Sabat, G., Santolaria, P., Ya´niz, J.L., Nogareda, C., De Rensis, F., and Lo´pez-Be´jar, M. 2007. Climate factors affecting conception rate of high producing dairy cows in northeastern Spain. Theriogen. 67: 1379–1385.
  18. Grummer, R.R., Mashek, D.G., and Hayirli, A. 2004. Dry matter intake and energy balance in the transition period.Vet. Clin. J. Food. Anim. 20: 447–470.
  19. Haslett, C., Savill, S. and Meagher, L. 1989. The neutrophil. Curr. J. Opin. Immun. 2: 10-18.
  20. Hayirli, A., Grummer, R.R., Nordheim, E.V. and Crump, P.M. 2002. Animal and dietary factors affecting feed intake during the prefresh transition period in Holsteins. J. Dairy. Sci. 85: 3430–3443.
  21. Heck, J.M.L., van Valenberg, H.J.F., Dijkstra, J. and van Hooijdonk, A.C.M. 2009. Seasonal variation in the Dutch bovine raw milk composition. J. Dairy. Sci. 92: 4745–4755.
  22. Machado, V.S., Oikonomou, G., Lima, S.F., Bicalhoa, M.L.S., Kacar, C., Foditsch, C., Felippeb, M.J., Gilbert, R.O. and Bicalho. R.C. 2014. The effects of injectable trace minerals (selenium, copper, zinc, and manganese) on peripheral blood leukocyte activity and serum superoxide dismutase activity of lactating Holstein cows. Vet. J. 200: 299–304.
  23. Matsui, T. 2012. Vitamin C nutrition in cattle. Asian-Aust. J. Anim. Sci. 25: 597 - 605.
  24. Muehlenbein, E.L., Brink, D.R., Deutscher, G.H., Carlson, M.P. and Johnson, A.B. 2001. Effect of inorganic and organic copper supplemented to first- calf cows on cow reproduction and calf health and performance. J. Anim. Sci. 79: 1650-1659.
  25. National Research Council. 2001. Nutrient Requirements of Dairy Cattle. 7th Revised Edition, National Academy of Sciences. Washington, D.C. 381 Pp.
  26. Rejeb, M., Raoudha, S. and Najar. T. 2016. Role of vitamin C on immune function under heat stress Condition in dairy cows. Asian J. Anim. Vet. Adv. 11: 717-724.
  27. Roche, J.R., Friggens, N.C., Kay, J.K., Fisher, M.W., Stafford K.J. and Berry, D.P. 2009. Invited review: Body condition score and its association with dairy cow productivity, health, and welfare. J. Dairy. Sci. 92: 5769–5801.
  28. Roche, J.R., Kay, J.K., Friggens, N.C., Loor, J.J. and Berry, D.P. 2013. Assessing and managing body condition score for the prevention of metabolic disease in dairy cows. J. Vet. Clin. Food Anim. Pract. 29: 323–336.
  29. Scaletti, R.W. and Harmon, R.J. 2012. Effect of dietary copper source on response to coliform mastitis in dairy cows. J. Dairy. Sci. 95: 654-662.
  30. Sharma, M.C., Joshi, C. and Gunjan. D. 2008. Therapeutic management of copper deficiency in buffalo heifers: Impact on immune function. J. Vet. Res. Commun. 32: 49–63.
  31. Solaiman, S.G., Maloney, M.A., Qureshi, M.A., Davis, G. and Andrea, G.D. 2001. Effect of high copper supplements on performance, health, plasma copper and Enzyme in goats. Small Rum. Res. 41: 127-139.
  32. Spears, J.W., Kegley, E.B., and Mullis, L.A. 2004. Bioavailability of copper from tribasic copper and copper sulfate in growing cattle. J. Anim. Feed. Sci. Technol. 116: 1-13.
  33. Suttle, N.F. 2010. Mineral Nutrition of Livestock. 4th Edition. CABI Publishing, Wallingford, Cambridge, UK. 579 Pp.
  34. Tan, X.Y., Luo, Z., Liu, X. and Xie, C.X. 2011. Dietary copper (Cu) requirement for juvenile yellow catfish Pelteobagrus fulvidraco. Aquac. Nut. 17: 170–176.
  35. Tao, S., Thompson, I.M., Monteiro, A.P.A., Hayen, M.J., Young L.J. and Dahl, G.E. 2012. Effect of cooling heat-stressed dairy cows during the dry period on insulin response. J. Dairy. Sci. 95: 5035–5046.
  36. Van Saun, R.J. 2016. Indicators of dairy cow transition risks: Metabolic profiling revisited. Tiera. Prax. Ausg. Gross. Nutz. 44: 118-126.
  37. Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy. Sci. 74: 3593-3597.
  38. Wankhade, P.R., Manimaran, A., Kumaresan, A., Jeyakumar, S., Ramesha, K.P., Sejian, V., Rajendran, D., and Varghese, M.R. 2017. Metabolic and immunological changes in transition dairy cows: A review : Vet. World. 10: 1367-1377.
  39. Ward, J.D., Spears, J.W. and Kegley, E.B. 1996. Bioavailability of copper proteinate and copper carbonate relative to copper sulfate in cattle. J. Dairy. Sci. 79: 127- 132.
  40. Weiss, W.P. 2001. Effeect of dietary vitamin C on concenteration of ascorbic acid in plasma and milk. J. Dairy. Sci. 84: 2302- 2307.
  41. Weiss, W.P. and Hogan, J.S. 2007. Effects of dietary vitamin C on neutrophil function and responses to intramammary infusion of lipopolysaccharide in periparturient dairy cows. J. Dairy. Sci. 90: 731–739.
  42. West, J.W. 2003. Effects of heat-stress on production in dairy cattle. J. Dairy. Sci. 86: 2131–2144.
  43. Wolf, G. 1993. Uptake of ascorbic acid by human neutrophils. Nutr. Rev. 51: 337-338.
  44. Yang, Z.B., Yang, W.R., Zhang, S.Z., Li, Z.Y. and Zhao, H. 2007. Effect of copper and zinc on blood and milk parameters and performance of dairy cows. J. Anim. Feed. Sci. 16: 571–575.