اثر مخلوط مکمل آلی کروم و روی با سیاهدانه بر فراسنجه‌های خونی ، ظرفیت آنتی اکسیدانی و عملکرد بره‌های سنجابی تحت استرس حمل و نقل

نوع مقاله: مقاله پژوهشی

نویسندگان

1 عضو هیات علمی دانشگاه رازی

2 گروه علوم دامی دانشگاه رازی

3 دانشگاه رازی

چکیده

سابقه و هدف: اهمیت بررسی استرسها در نشخوارکنندگان در حفظ سلامتی و افزایش ظرفیت تولیدی آنها می باشد . نمود استرس در دامها با تغییراتی درپارامترهای فیزیولوژیکی و عملکردی مشخص می‌گردد. دوره های طولانی حمل ونقل با وسایل نقلیه بویژه در آب و هوای نامطلوب و یا ازدحام دام‌ها در کامیون‌هایویژه حمل و نقل منجر به خستگی، عدم آسایش، کاهش وزن و افزایش شیوع بیماریهای عفونی می‌گردد. شرایط تنش‌زای مختلفی سبب افزایش تولید رادیکال‌های آزاد و در نتیجه استرس اکسیداتیو می‌شود. جیره‌های حاوی سطوح مناسب از آنتی اکسیدان‌ها، با مهار رادیکال های آزاد منجر به بهبود اثرات مضر استرس می‌شود. مطالعات نشان داده‌اند استفاده از مواد معدنی و گیاهان دارویی با خاصیت آنتی اکسیدانی در تغذیه دام، شرایط فیزیولوژیکی دام را بهبود بخشیده و بواسطه تعدیل پرواکسیدان ها- آنتی اکسیدان ها، از افت ظرفیت تولیدی ممانعت می‌نماید. تحقیق حاضر جهت مقایسه اثر مکمل آلی کروم و روی با مخلوط سیاهدانه بر پیشگیری از استرس و بهبود عملکرد بره ها انجام شد.
مواد و روش‌ها: هیجده رأس بره نر نژاد سنجابی 24-26 هفته با وزن اولیه 2±32 کیلوگرم به مدت 30 روز به طور تصادفی در سه گروه 6 راسی مورد بررسی قرار گرفتند و بصورت انفرادی نگهداری شدند. تیمارها شامل گروه 1:تیمار شاهد (جیره پایه)؛ گروه 2: خوراک پایه + 30 گرم سیاه دانه‌/ کیلوگرم ماده خشک جیره+ 1 میلی گرم مکمل آلی کروم /کیلو گرم ماده خشک وگروه 3: خوراک پایه + 30 گرم سیاه دانه‌/ کیلوگرم ماده خشک جیره+ 50 میلی گرم مکمل آلی روی /کیلو گرم ماده خشک جیره‌، مورد مطالعه قرار گرفتند. در طول دوره آزمایش؛ عملکرد بره‌ها بررسی و نمونه‌برداری از فراسنجه‌های خونی انجام گرفت. حمل و نقل بره ها پس از دو هفته از شروع آزمایش و به مدت 3 ساعت انجام شد. جهت بررسی فراسنجه های خونی و آنتی اکسیدانی سرم نمونه های خون ( 10 میلی لیتر ) از رگ گردنی بره‌ها قبل از حمل و نقل (BT)، بلافاصله بعد از حمل و نقل (AT1) و 24 ساعت بعد از حمل و نقل (AT2) گرفته شد.
یافته‌ها: اثر تیمارهای آزمایشی بر اضافه وزن روزانه معنی‌دار بود(05/0>P). مقدار مصرف خوراک در دامهای تیمار سیاهدانه+روی بیش از تیمارهای دیگر بود، در حالی که در تیمار سیاهدانه+ کروم ضریب تبدیل خوراک نسبت به گروه شاهد کاهش یافت (05/0>P). اثر تیمارهای آزمایشی بر سطح گلوکز، کورتیزول، آلبومین، لاکتات دهیدروژناز سرم خون نیز در طول دوره استرس حمل ونقل(AT1) معنی‌دار بود (05/0>P). اثر تیمارآزمایشی سیاهدانه + کروم بر میزان مالون دی آلدئید مثبت و معنی‌دار بود (05/0>P).
نتیجه‌گیری: با توجه به نتایج این آزمایش افزودن مکمل سیاهدانه همراه با کروم و یا روی از دو هفته قبل از حمل و نقل دام در جیره‌ سبب بهبود عملکرد و کاهش استرس در طول حمل و نقل بره‌ها شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of mixed Blackseed with chromium-methionine or zinc-methionine supplementations on serum components and physiological responses inُ Sanjabi lambs during road transportation

نویسندگان [English]

  • soheilla kaki 2
  • fardin hozhabri 3
  • zahra nikosefat 3
2 animal science department, razi university
3 animal science department, razi university
چکیده [English]

Background and objectives: Stress in livestock is characterized by changes in physiological and functional parameters. The importance of stress in ruminants is in maintaining health and increasing their productive capacity. Long periods of transport with vehicles, especially in adverse weather conditions, resulted in fatigue, lack of comfort, weight loss and an increase in the prevalence of infectious diseases. Different stress conditions increase the production of free radicals species and thus oxidative stress. Diets containing appropriate levels of antioxidants, by inhibiting free radicals, improve the harmful effects of stress. Studies have shown that the use of minerals and medicinal plants with antioxidant properties in livestock feed improves the physiological conditions and preventing production capacity by modifying antioxidants- pro-oxidants. The present study was conducted to compare the effect of chromium- methionine or zinc-methionine supplementations with black seed on transported stress and improve the livestock performance.
Materials and methods: In this study, 18 Sanjabi lambs (24-26 weeks age) were randomly assigned to three dietary treatments two weeks before road transportation: (i) basal diet (control); (ii) basal diet with added 30 grams black seed + 50mg Zn-Met /Kg DM; and (iii) with added 30 grams black seed +1mg Cr-Met/Kg DM. During the experimental period, lambs performance and changes on blood parameters were performed. Lambs were transported after two weeks of starting the experiment for 3 hours. Blood samples were collected via jugular puncture before transportation (BT), after transportation (AT1), as well as 24h after transportation (AT2). Blood samples were placed on ice at once after collection and centrifuged at 3500 × g for 15 min at 4°C for serum harvesting. The serum was maintained at -20 °C until analysis.
Results: Lambs fed on black seed +Zn-Met or black seed +Cr-Met diets had higher feed intake and average daily gain (ADG) than those of control (P<0.05). Lambs fed on black seed +Zn-Met diet had higher feed intake compared with the control group (P<0.05), while lambs fed on the black seed +Cr-Met diet showed lower feed conversion ratio (FCR) than that of the controls (P<0.05). The supplemented lambs on glucose, cortisol, albumin, lactate dehydrogenase (LDH) concentrations at AT1 than those of control (P<0.05). Lambs fed on black seed +Cr-Met diet had lower blood malondialdehyde (MDA) at AT1 when compared with the control (P<0.05).
Conclusion: It can be concluded that incorporation of black seed +Zn-Met or black seed +Cr-Met to the diet can reduce the adverse effects of transportation stress in lambs.

کلیدواژه‌ها [English]

  • cortisol
  • malondialdehyde
  • Antioxidant
  • herbal medicinal
  • Minerals
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