تاثیر دوزهای مختلف eCG بر عملکرد تولید مثلی میش‌های ترکی قشقایی در فصل پائیز

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه علوم دامی-دانشکده کشاورزی - دانشگاه یاسوج

2 هیات علمی

چکیده

چکیده
سابقه و هدف: تولیدمثل مهمترین صفت اقتصادی در پرورش گوسفند است که می‌توان آن را با استفاده از روش‌های مختلف از جمله هورمون درمانی دست‌کاری کرد. از هورمون‌های مهم که به‌طور وسیعی برای بهبود باروری در گوسفند استفاده می‌شود eCG است. با این وجود، در برخی از پژوهش‌ها استفاده از دوزهای زیاد یا کم این هورمون سبب افزایش تعداد و رشد فولیکول‌های بزرگ شده، و با وجود افزایش نرخ تخمک‌ریزی باعث کاهش نرخ آبستنی شده است. از آنجایی‌که، تاکنون گزارشی مبنی بر تاثیر eCG بر عملکرد تولیدمثلی میش‌های ترکی قشقایی ارائه نشده است پژوهش حاضر به‌منظور بررسی تاثیر دوزهای مختلف eCG بر نرخ پاسخ فحلی، زمان آغاز فحلی، نرخ آبستنی و دوقلوزایی این میش‌ها در فصل پائیز، طراحی و اجرا شد.

مواد و روش‌ها: در این مطالعه 75 راس میش ترکی قشقایی با سن 3 تا 4 سال و میانگین وزنی 6/357 کیلوگرم انتخاب و به‌طور تصادفی به 5 گروه آزمایشی تقسیم شدند. چرخه فحلی تمام میش‌ها با استفاده از اسفنج‌های حاوی پروژسترون در فصل پائیز در یک دوره 12 روزه همزمان‌سازی شد. یک روز قبل از برداشت اسفنج، به گروه‌های دوم، سوم، چهارم و پنجم به‌ترتیب 300، 400، 500 و 600 واحد بین‌المللی eCG به‌صورت درون ماهیچه‌ای تزریق، و گروه اول (بدون تزریق eCG) به‌عنوان شاهد در نظر گرفته شد. فراسنجه‌های مورد بررسی در این پژوهش شامل: زمان آغاز فحلی(ساعت)، نرخ پاسخ به فحلی، نرخ آبستنی، نرخ دوقلوزایی، تعداد بره‌های متولد شده، نرخ بره‌زایی و نرخ چندقلوزایی بود.

یافته‌ها: نرخ پاسخ به فحلی در گروه‌های دریافت کننده eCG، 100 درصد بود که به‌طور معنی‌داری (01/0P<) نسبت به گروه شاهد (80 درصد) بیشتر بود. نتایج نشان داد که زمان آغاز فحلی پس از برداشت اسفنج در گروه چهارم و پنجم (به‌ترتیب 03/36 و 68/32 ساعت) به‌طور معنی‌داری (05/0P<) نسبت به گروه‌های شاهد، دوم و سوم زودتر بود. اگر چه درصد میش‌های آبستن در گروه‌های آزمایشی اختلاف معنی‌داری نداشت (05/0<P) با این وجود در گروه پنجم (600 واحد بین‌المللی eCG) نسبت بیشتری از میش‌ها آبستن شدند (3/93 درصد) در حالی که در گروه شاهد این نسبت 3/83 درصد بود. نتایج نشان داد که تمام گروه‌های دریافت کننده eCG نسبت به گروه شاهد دارای نرخ دوقلوزایی بیشتری بودند و از طرف دیگر با افزایش دوز eCG تعداد میش‌های دوقلوزا افزایش پیدا کرد. نتایج این پژوهش نشان داد که نرخ‌های بره‌زایی و چندقلوزایی در تیماری که میش‌ها همراه با برنامه همزمان‌سازی فحلی دوز 600 واحد بین‌المللی eCG را دریافت نمودند نسبت به تیمار شاهد و میش‌های دریافت کننده 300 و 400 واحد بین‌المللی eCG بیشتر بود.

نتیجه‌گیری: به‌طور کلی نتایج نشان داد که دوز 600 واحد بین‌المللی eCG دارای بیشترین تاثیر در افزایش نرخ آبستنی و دوقلوزایی در میش‌های ترکی قشقایی در فصل پائیز بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effects of different eCG doses on reproductive performance in Torki-Ghashghaei ewes in autumn season

چکیده [English]

Abstract1
Background and objectives: Reproductive performance is the most important economic trait in sheep production and it can be manipulated by using hormonal treatments. Equine chorionic gonadotropin (eCG) is widely used for improving the fertility in ewes. In some studies the use of low and high doses of this hormone increased number and the size of large follicles and also it could enhance the ovulation rate, but it decreased pregnancy rate. As it was not found any report about the effect of using eCG on reproductive performance of Torki-Ghashghaei ewes, this study was conducted to investigate the effect of different dose of eCG on estrus response, estrus onset, pregnancy rate and twining rate of these ewes in autumn season.

Materials and methods: In this study, the total of 75 cyclic ewes with the mean weight of 573.6 kgs were randomly assigned to five experimental groups (n=15/group). The estrous cycle of all ewes was synchronized with the insertion of intravaginal progesterone sponges during a 12 days period. One day before withdrawaling progesterone sponges 300, 400, 500 and 600 IU eCG were injected intramuscularly to ewes in group Π, Ш, ΙV and V, respectively and the group Ι (without eCG injection) was used as control group. The reproductive variables such as: time of estrus onset (hour), estrous response, pregnancy rate, twining rate, fecundity and prolificacy rate were measured in this experiment.

Results: Estrous responses for all eCG recipient ewes (100%) were significantly higher (P<0.01) than the control group (80%). The results showed that, after withdrawaling of sponges the estrus onset in ΙV and V groups (36.03 and 32.68 h, respectively) were significantly (P<0.05) earlier than groups Π, Ш and the control group. Although there were not any significant differences among the treatment groups in the percentage of pregnancy, this parameter in group V (93.3%) was numerically more than control group (83.3%). The results also showed that all eCG recipient groups had higher twining rates compared with control group and increasing the dose of eCG increased the twining rate in ewes. The results of this study indicated that in synchronized ewes which were received 600 IU eCG had more fecundity and prolificacy rate compared with the control group and also the groups received 300 and 400 IU eCG.

Conclusion: Generally, the results showed that injecting 600 IU eCG had the most effect on increasing pregnancy rate and twining rate in Torki-Ghashghaei ewes in autumn season.

کلیدواژه‌ها [English]

  • Keywords: Torki-Ghashghaei ewe
  • eCG
  • Reproductive performance
  • Twining rate
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